Primed uterine-derived regenerative cell compositions and uses thereof

A heterogeneous IFN-gamma primed cell composition comprising:

• a population of feline or canine uterine-derived mesenchymal progenitor cells;
• and
• a population of feline or canine uterine-derived epithelial progenitor cells;
• wherein the mesenchymal progenitor cells and epithelial progenitor cells are co-cultured;
• wherein the mesenchymal progenitor cells and epithelial progenitor cells are in a ratio of 20% to 80%, 40% to 60%, 50% to 50%, 60% to 40%, 80% to 20%, about 20% to about 80%, about 40% to about 60%, about 50% to about 50%, about 60% to about 40%, or about 80% to 20%, or a ratio within a range defined by any two of the aforementioned percentages.

A method of preparing a heterogeneous cell composition, comprising:

• contacting a cell suspension comprising IFN-gamma primed feline or canine uterine tissue derived mesenchymal progenitor cells and IFN-gamma primed feline or canine uterine tissue derived epithelial progenitor cells with recombinant FGF-2;
• and
• culturing the cell suspension with the recombinant FGF-2 for 3, 4, 5, or 6 passages, days, or culture intervals to produce the heterogeneous cell composition;
• wherein the heterogeneous cell composition comprises mesenchymal progenitor cells and epithelial progenitor cells in a ratio of 20% to 80%, 40% to 60%, 50% to 50%, 60% to 40%, 80% to 20%, about 20% to about 80%, about 40% to about 60%, about 50% to about 50%, about 60% to about 40%, or about 80% to 20%, or a ratio within a range defined by any two of the aforementioned percentages.

A method of preparing a heterogeneous cell composition, comprising:

• contacting a cell suspension comprising IFN-gamma primed feline or canine uterine-derived V+/C− cells and V+/C+ cells with recombinant FGF-2;
• and
• culturing the cell suspension with the recombinant FGF-2 for 3, 4, 5, or 6 passages, days, or culture intervals to produce the heterogeneous cell composition;
• wherein the heterogeneous cell composition comprises V+/C− cells and V+/C+ cells in a ratio of 20% to 80%, 40% to 60%, 40% to 50%, 50% to 40%, 60% to 40%, 80% to 20%, about 20% to about 80%, about 40% to about 60%, about 40% to about 50%, about 50% to about 40%, about 60% to about 40%, or about 80% to about 20%, or a ratio within a range defined by any two of the aforementioned percentages.