Method for Obtaining Cultures of Mesenchymal Stem Cells (MSCs) from the Perivascular Space of the Umbilical Cord Vessel

A method for obtaining mesenchymal stem cell culture from the perivascular space around the umbilical cord blood vessel, wherein the method comprises

• cutting out a section of umbilical cord;
• filling the umbilical cord vessel with 0.01-0.5% collagenase solution;
• sealing both ends of the umbilical cord and incubating the umbilical cord at 37° C. for 15-45 minutes for first incubation;
• washing the vessel with a first balanced normal saline solution and harvesting the first balanced salt solution;
• centrifuging the first balanced salt solution;
• filling the vessel with a new 0.01-0.5% collagenase solution;
• sealing the two ends of the umbilical cord again and incubating the umbilical cord at 37° C. for 25-65 minutes for second incubation;
• washing the vessel with a second balanced salt solution and harvesting the second balanced salt solution;
• and
• centrifuging the harvested first and second balanced salt solution and culturing the pellet obtained from centrifugation on a selective medium.