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US 2025/0019658 A1Published application
Maturation medium for pluripotent stem cell-derived cardiomyocytes
AssigneeThe Governing Council of the University of Toronto
InventorsNeal I. CALLAGHAN, Craig A. SIMMONS
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Abstract
A culture medium is provided for the maturation of pluripotent stem-cell derived cardiomyocytes. The culture medium contains a number of soluble factors, which can include putrescine, leukemia inhibitor factor and metformin.
Claims30
An aqueous cell culture medium comprising.
- a high-flux carbon source or central carbon metabolism inhibitor;
- at least one secondary metabolite;
- at least one of putrescine, leukemia inhibitory factor (LIF) and metformin.
A composition for improving the cardiomyocyte maturing properties of a cell culture medium, the composition comprising one or more of putrescine, leukemia inhibitory factor (LIF) and metformin.
Dependencies are inferred from the claim text.