Method for producing milk like products
AssigneeSociete des Produits Nestle S.A.
InventorsMARIA MARQUES DE LIMA, VIRGINIE BACHMANN, ARIANNA BIANCHI, OMID MASHINCHIAN, MARINE KRAUS
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Abstract
A method for producing mammary gland cells and a method of producing a mammalian milk like product, for example a human milk like product comprising generating lactocytes derived from mammalian induced pluripotent stem cells (miPSC), for example human induced pluripotent stem cells (hiPSC), and expressing the mammalian milk like product, for example the human milk like product from lactocytes.
Claims22
A method of producing a population of mammary gland cells, comprising:
- i) culturing mammalian induced pluripotent stem cells (miPSCs) in a culture medium comprising bone morphogenic protein 4 (BMP4) to generate embryoid bodies (EBs), and
- ii) growing the EBs to generate a population of mammary cells.
(canceled)
A method for producing a mammalian milk like product, comprising:
- C) Generating lactocyte mammary-like gland organoids derived from mammalian induced pluripotent stem cells (miPSC);
- D) Secreting the mammalian milk like product from said lactocytes,
- wherein step A) comprises culturing the miPSCs in a culture medium comprising BMP4.
22-27. (canceled)
Dependencies are inferred from the claim text.