Production method for sheet-like retinal tissue

A method for manufacturing retinal tissue having an epithelial structure, comprising

• suspension-culturing or adhesion-culturing a dispersed retinal cell population in a culture medium comprising a Wnt signaling pathway agonist,
• wherein the retinal cell population comprises one or more cells selected from the group consisting of a retinal progenitor cell and a photoreceptor progenitor cell,
• wherein the culture medium further comprises one or more substances selected from the group consisting of a ROCK inhibitor, a SHH signaling pathway agonist and an FGF signaling pathway agonist, and
• wherein the method further comprises (i) differentiating pluripotent stem cells to obtain a cell aggregate comprising one or more cells selected from the group consisting of the retinal progenitor cell and the photoreceptor progenitor cell, and (ii) dispersing the cell aggregate comprising one or more cells selected from the group consisting of a retinal progenitor cell and a photoreceptor progenitor cell to obtain the dispersed retinal cell population.