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US 2025/0019647 A1Published application
Expansion of stem cells in hollow fiber bioreactors
AssigneeHealios K.K.
InventorsJozef Albert Martha PINXTEREN, David CRAEYE
Life of this patent
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Abstract
The invention is directed to producing large numbers of cells using hollow fiber bioreactor technology. The cells are non-embryonic stem, non-germ cells that can be characterized by one or more of the following: extended replication in culture and markers of extended replication, such as telomerase, markers of pluripotentiality, and broad differentiation potential, without being transformed.
Claims14
A method of expanding cells ex vivo, the method comprising the steps of:
- a) seeding the cells on a hollow fiber substrate so that the cells adhere to the substrate;
- b) expanding the adhered cells on the substrate;
- and c) removing the expanded cells from the substrate, wherein the cells are non-embryonic stem, non-germ cells, wherein the cells express telomerase, are not transformed, are devoid of mesenchymal stem cells, and have a normal karyotype, and wherein, prior to seeding, the cells have undergone 10-40 cell doublings, and wherein the hollow fiber substrate is in a closed continuous perfusion bioreactor.
Dependencies are inferred from the claim text.