Cell culture medium and supplements for cellular meat production
OwnerChe John CONNON, Ricardo GOUVEIAinventors
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Abstract
The present invention provides novel cell culture media and supplements for use in serum-free or reduced-serum cell culture. Corresponding methods and uses are also provided. The novel cell culture media and supplements are particularly beneficial when used during in vitro cell culture of fat cells, muscle cells, or a combination thereof.
Claims18
Use of a serum-free or reduced-serum cell culture medium comprising a basal medium and one or more macromolecular crowding agents for in vitro cell culture, wherein:
- a) the cell is a muscle cell and the macromolecular crowding agent is selected from the group consisting of:
- PVP40, Carrageenan, PEG8, PVP360, PEG35, Ficoll® 70, and Ficoll® 400;
- or a combination thereof;
- or
- b) the cell is a fat cell and the macromolecular crowding agent is selected from the group consisting of:
- PEG8, PEG35, PVP40, PVP360 and carrageenan;
- or a combination thereof;
- wherein reduced-serum cell culture medium has a maximum of 0.1% (v/v) serum in the cell culture medium.
An in vitro serum-free or reduced-serum cell culture method, comprising culturing cells in a serum-free or reduced-serum cell culture medium comprising a basal medium and one or more macromolecular crowding agents, wherein:
- a) the cells are muscle cells and the macromolecular crowding agent is selected from the group consisting of:
- PVP40, carrageenan, PEG8, PVP360, PEG35, Ficoll® 70, and Ficoll® 400;
- or a combination thereof;
- or
- b) the cells are fat cells and the macromolecular crowding agent is selected from the group consisting of:
- PEG8, PEG35, PVP40, PVP360 and carrageenan;
- or a combination thereof
- wherein reduced-serum cell culture medium has a maximum of 0.1% (v/v) serum in the cell culture medium.
A serum-free or reduced-serum cell culture medium for in vitro cell culture, wherein the cell culture medium comprises a basal medium and one or more macromolecular crowding agents selected from the group consisting of:
- PVP40, PEG8, PVP360 and PEG35;
- wherein reduced-serum cell culture medium has a maximum of 0.1% (v/v) serum in the cell culture medium.
The cell culture medium, use or method according to any preceding claim, wherein the cells are a combination of muscle cells and fat cells and the macromolecular crowding agent is selected from the group consisting of:
- PEG8, PEG35, PVP40, PVP360 and carrageenan;
- or a combination thereof.
The cell culture medium, use or method according to any preceding claim, wherein the basal medium is DMEM/F12.
The cell culture medium, use or method according to any preceding claim, wherein the cell culture medium is a serum-free cell culture medium, optionally wherein the cell culture medium does not contain materials obtained from an animal.
The cell culture medium, use or method according to any preceding claim, wherein the cell culture medium further comprises glutamine, optionally wherein the cell culture medium further comprises ascorbic acid, insulin, transferrin, selenium, and ethanolamine.
The cell culture medium, use or method according to any preceding claim, wherein the cell culture medium further comprises penicillin and streptomycin.
A cell culture medium supplement for in vitro serum-free or reduced-serum cell culture, comprising one or more macromolecular crowding agents selected from the group consisting of:
- PVP40, PEG8, PVP360, and PEG35;
- or a combination thereof, wherein the supplement further comprises:
- insulin, transferrin, selenium, ethanolamine, ascorbic acid and/or glutamine.
A hermetically-sealed vessel containing a serum-free or reduced-serum cell culture medium or a cell culture medium supplement according to any of the preceding claims.
Dependencies are inferred from the claim text.