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US 2025/0019644 A1Published application
Quantitatively Mapping Amino Acid Properties (QMAP) Cell Culture Media
AssigneeUniversity of Iowa Research Foundation
InventorsEric TAYLOR, Jane BUCHANAN
Life of this patent
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Abstract
In certain embodiments, the present invention provides Qualitatively Mapping Amino Acid Properties (QMAP) cell culture media comprising an amino acid component, wherein the amino acids are present in the component at uniform concentrations; an inorganic salt component; an RPMI 1640 Vitamin Solution component; and auxiliary ingredient component. In certain aspects, the present invention also provides methods of making the QMAP cell culture media and using the QMAP cell culture media to culture cells.
Claims16
A Qualitatively Mapping Amino Acid Properties (QMAP) cell culture media composition comprising:
- (a) an amino acid component comprising glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, and optionally glutamine and/or asparagine;
- wherein the amino acids are present in the component at uniform concentrations;
- (b) an inorganic salt component comprising Calcium Chloride (CaCl2 anhydrous), Magnesium Sulfate (MgSO4 anhydrous), Potassium Chloride (KCl), Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), and/or Sodium Phosphate monobasic (NaH2PO4—H2O);
- (c) an RPMI 1640 Vitamin Solution component comprising Biotin, Choline chloride, D-Calcium pantothenate, Folic Acid (folate), Niacinamide, Pyridoxine hydrochloride, Riboflavin, Thiamine hydrochloride, and/or Vitamin B12,;
- and
- optionally Ammonium chloride, i-Inositol and/or Para-aminobenzoic acid (PABA);
- (d) auxiliary ingredient component comprising D-Glucose, Sodium pyruvate, 3-Hydroxybutyric acid sodium salt, L-Carnitine hydrochloride, Sodium L-lactate, Penicillin-streptomycin, and/or Dialyzed fetal bovine serum. and
- (e) optionally, minerals and metals.
5 more elements
A Qualitatively Mapping Amino Acid Properties (QMAP) cell culture media composition comprising:
- (a) an amino acid component comprising glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, and optionally glutamine and/or asparagine;
- wherein the amino acids are present in the component at uniform concentrations;
- (b) an inorganic salt component comprising Calcium Chloride (CaCl2 anhydrous), Magnesium Sulfate (MgSO4 anhydrous), Potassium Chloride (KCl), Sodium Chloride (NaCl), and/or Sodium Phosphate monobasic (NaH2PO4—H2O);
- (c) an RPMI 1640 Vitamin Solution component comprising Biotin, Choline chloride, D-Calcium pantothenate, Folic Acid (folate), Niacinamide, Pyridoxine hydrochloride, Riboflavin, Thiamine hydrochloride, and/or Vitamin B12,;
- and
- optionally Ammonium chloride, i-Inositol and/or Para-aminobenzoic acid (PABA);
- (d) auxiliary ingredient component comprising D-Glucose, Sodium pyruvate, 3-Hydroxybutyric acid sodium salt, L-Carnitine hydrochloride, Sodium L-lactate, Penicillin-streptomycin, and/or Dialyzed fetal bovine serum. and
- (e) optionally, minerals and metals.
5 more elements
A Qualitatively Mapping Amino Acid Properties (QMAP) cell culture media composition comprising:
- (a) a pool of amino acids, wherein the pool of amino acids consists of:
- (1) alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (2) glycine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (3) glycine, alanine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (4) glycine, alanine, arginine, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (5) glycine, alanine, arginine, aspartic acid, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (6) glycine, alanine, arginine, aspartic acid, cysteine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (7) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (8) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (9) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (10) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (11) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (12) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, proline, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (13) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (14) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (15) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, tryptophan, tyrosine, valine, glutamine and asparagine;
- (16) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tyrosine, valine, glutamine and asparagine;
- (17) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, valine, glutamine and asparagine;
- (18) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, glutamine and asparagine;
- (19) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, and asparagine;
- (20) glycine, alanine, arginine, aspartic acid, cysteine, glutamic acid, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, serine, threonine, tryptophan, tyrosine, valine, and glutamine;
- wherein the amino acids are present in the pool at uniform concentrations;
- (b) an inorganic salt component comprising Calcium Chloride (CaCl2 anhydrous), Magnesium Sulfate (MgSO4 anhydrous), Potassium Chloride (KCl), Sodium Bicarbonate (NaHCO3), Sodium Chloride (NaCl), and/or Sodium Phosphate monobasic (NaH2PO4—H2O);
- (c) an RPMI 1640 Vitamin Solution component comprising Biotin, Choline chloride, D-Calcium pantothenate, Folic Acid (folate), Niacinamide, Pyridoxine hydrochloride, Riboflavin, Thiamine hydrochloride, and/or Vitamin B12,;
- and
- optionally Ammonium chloride, i-Inositol and/or Para-aminobenzoic acid (PABA);
- (d) auxiliary ingredient component comprising D-Glucose, Sodium pyruvate, 3-Hydroxybutyric acid sodium salt, L-Carnitine hydrochloride, Sodium L-lactate, Penicillin-streptomycin, and/or Dialyzed fetal bovine serum, and
- (e) optionally, minerals and metals.
25 more elements
Dependencies are inferred from the claim text.