Compositions and methods for producing fermented dairy products for storage at ambient temperature
AssigneeInternational N&H Denmark Aps
InventorYuejian Mao
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Abstract
Provided herein are compositions and methods for producing fermented dairy products that can be stored at ambient temperature. The compositions and methods provided herein include lactose-deficient bacteria capable of maintaining viability and/or pH when added to fermented dairy products having low levels of galactose and stored at ambient temperature. Also provided are fermented dairy products, e.g., yogurts, fermented dairy beverages, containing said lactose-deficient bacteria for storage at ambient temperature.
Claims15
A method of producing a fermented dairy product for storage at ambient temperature, comprising inoculating an initial fermented dairy product with one or more lactose-deficient bacterial strains, wherein the initial fermented food product comprises between about 0% and about 1% galactose.
A culture or kit of parts comprising a lactose-deficient bacterial strain, wherein the lactose deficient bacterial strain has a lactose metabolic activity of about or less than about 50%, 40%, 30%, 20%, or 10% or no lactose metabolic activity compared to a lactose metabolic activity of a lactose-metabolizing bacteria comprising a beta-galactosidase protein having the amino acid sequence encoded by the sequence of nucleotides set forth by SEQ ID NO:1.
A method for manufacturing a lactose-deficient bacterial strain comprising:
- a) providing a lactic acid bacterial strain as the mother strain, such as Lactiplantibacillus plantarum DSM 33121;
- b) introducing one or more mutations in the sequence of nucleotides of the promoter region of the beta-galactosidase gene lacL/M (PlacLM), such as the promoter region defined by any one of SEQ ID NO:10 or SEQ ID NO:11 of the strain;
- and
- c) screening for a lactose-deficient bacterial strain have a reduced or complete inability to metabolize lactose.
Dependencies are inferred from the claim text.