Culture method for high-concentration haematococcus pluvialis

A culture method for high-concentration Haematococcus pluvialis, comprising the following steps:

• 1) inoculating a Haematococcus pluvialis algae into a first culture medium for culturing for 7-10 days, collecting algal cells, inoculating the algal cells into a new first culture medium for culturing until a dry weight concentration of the algal cells in a Haematococcus pluvialis liquid obtained by culturing is equal to or greater than 2.0 g/L, and collecting inoculated algal cells as motile vegetative cells,
• wherein the first culture medium is a first improved Bold's Basal Medium (BBM) culture medium, and a concentration of NaNO3 in the first improved BBM culture medium is 1.3-1.7 g/L, and an illumination intensity during the culturing of the motile vegetative cells is controlled at 30-100 μmol/m2 by aid of a light conversion film, wherein the light conversion film comprises an aluminum-based non-rare earth oxide type inorganic fluorescent powder to convert white light into red light;
• 2) inoculating the motile vegetative cells obtained in step 1) into a second culture medium for culturing for 13-15 days to obtain nonmotile vegetative cells with aged cell walls,
• wherein the second culture medium is a simplified BBM culture medium, and the simplified BBM culture medium does not comprise NaNO3, and an illumination intensity during the culturing of the nonmotile vegetative cells is controlled at 100-125 μmol/m2 by aid of a light conversion film, wherein the light conversion film comprises an aluminum-based non-rare earth oxide type inorganic fluorescent powder to convert white light into red light;
• 3) inoculating the nonmotile vegetative cells obtained in step 2) into a third culture medium for culturing for 10-14 days, inducing a generation of astaxanthin until a content of the astaxanthin in an algal powder is equal to or greater than 6%, and finishing the culturing,
• wherein the third culture medium is a second improved BBM culture medium, and a concentration of NaCl in the second improved BBM culture medium is 1.3-1.7 g/L, and an illumination intensity during induction of the generation of the astaxanthin is controlled at 125-300 μmol/m2 by aid of a light conversion film, wherein the light conversion film comprises an aluminum-based non-rare earth oxide type inorganic fluorescent powder to convert white light into red light.