Method for obtaining sulfated nanoparticles of cellulose and mannan from endosperms

A method for obtaining sulfated nanoparticles of cellulose, or sulfated nanoparticles of mannan, or a combination thereof, from endosperms, comprising:

• (a) acquiring a ground flour of an endosperm of a seed, nut, or pit, wherein the ground flour contains polysaccharides, and wherein the polysaccharides contain either cellulose or mannan, or both cellulose and mannan;
• (b) pretreating the ground flour to condition the polysaccharides for hydrolysis and functionalization thereof;
• (c) preparing a reagent for hydrolysis and functionalization of the polysaccharides, wherein the reagent comprises a solution of glycerol, water, and sulfuric acid at predetermined concentrations;
• (d) adding the reagent to the pretreated polysaccharides to form a suspension, wherein adding the reagent to the suspension converts either the cellulose into nanoparticles or the mannan into nanoparticles, or converts both the cellulose and the mannan into nanoparticles, and wherein the nanoparticles are sulfated by the reagent;
• (e) stopping the hydrolysis and functionalization reaction;
• (f) separating either the sulfated nanoparticles of cellulose or the sulfated nanoparticles of mannan from the suspension, or separating both the sulfated nanoparticles of cellulose and the sulfated nanoparticles of mannan from the suspension;
• (g) washing the nanoparticles;
• (h) creating a stable colloidal suspension of the sulfated nanoparticles;
• (i) adjusting the pH of the colloidal suspension to between 3.0 and 8.0;
• and
• (j) homogenizing the stable colloidal suspension.