Chimeric antigen receptor
AssigneeAutolus Limited
InventorsMartin Pulé, Evangelia Kokalaki, Shaun Cordoba, Shimobi Onuoha, Simon Thomas, Biao Ma, Mathieu Ferrari
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Abstract
The present invention provides a chimeric antigen receptor (CAR) which binds a target antigen having a bulky extra-cellular domain, wherein the CAR comprises a Fab antigen binding domain. The present invention also provides nucleic acid sequences and constructs encoding such a CAR, cells expressing such a CAR and their therapeutic uses.
Claims12
1-25. (canceled)
A method for killing a CD22-expressing target cell in a subject, the method comprising the step of administering to the subject a cell which expresses a chimeric antigen receptor (CAR) comprising an anti-CD22 antigen-binding domain which comprises:
- a) a heavy chain variable region (VH) having complementarity determining regions (CDRs) with the following sequences:
- (SEQ ID No. 93)
- CDR1 - NFAMA
- (SEQ ID No. 94)
- CDR2 - SISTGGGNTYYRDSVKG
- (SEQ ID No. 95)
- CDR3 - QRNYYDGSYDYEGYTMDA;
- and
- b) a light chain variable region (VL) having complementarity determining regions (CDRs) with the following sequences:
- (SEQ ID NO:
- 96)
- CDR1 - RSSQDIGNYLT,
- (SEQ ID NO:
- 97)
- CDR2 - GAIKLED,
- and
- (SEQ ID NO:
- 98)
- CDR3 - LQSIQYP.
A method for making a cell, which comprises the step of introducing into a cell a nucleic acid sequence encoding an anti-CD22 chimeric antigen receptor (CAR) which comprises an antigen-binding domain which comprises:
- a) a heavy chain variable region (VH) having complementarity determining regions (CDRs) with the following sequences:
- (SEQ ID No. 93)
- CDR1 - NFAMA
- (SEQ ID No. 94)
- CDR2 - SISTGGGNTYYRDSVKG
- (SEQ ID No. 95)
- CDR3 - QRNYYDGSYDYEGYTMDA;
- and
- b) a light chain variable region (VL) having complementarity determining regions (CDRs) with the following sequences:
- (SEQ ID No. 96)
- CDR1 - RSSQDIGNYLT
- (SEQ ID No. 97)
- CDR2 - GAIKLED
- (SEQ ID No. 98)
- CDR3 - LQSIQYP
Dependencies are inferred from the claim text.