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US 2025/0019429 A1Published application
Methods for Producing Anti-TNF Antibody Compositions
AssigneeJanssen Biotech, Inc.
InventorsKristopher Barnthouse, Subinay Ganguly, Maarten Groeneveld, Manuel Lopez, JR., Michael Nedved, Kevin D. Smith
Life of this patent
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Abstract
Presented here are methods for producing a recombinant anti-TNF antibody having a heavy chain (HC) comprising SEQ ID NO:38 and a light chain (LC) comprising SEQ ID NO:37 and compositions comprising the recombinant anti-TNF antibody.
Claims20
Anti-TNF antibodies comprising:
- (i) a heavy chain comprising an amino acid sequence of SEQ ID NO:38;
- and (ii) a light chain comprising an amino acid sequence of SEQ ID NO:37, wherein the anti-TNF antibodies are expressed in Chinese Hamster Ovary cells (CHO cells), wherein the oligosaccharide profile of the anti-TNF antibodies comprises:
- a) total neutral oligosaccharide species >99.0%;
- b) total charged oligosaccharide species <1.0%;
- c) individual neutral oligosaccharide species G0F>60.0%;
- d) individual neutral oligosaccharide species G1F<20.0%;
- or
- e) individual neutral oligosaccharide species G2F<5.0%.
5 more elements
A method of manufacture for producing anti-TNF antibodies comprising:
- (i) a heavy chain comprising an amino acid sequence of SEQ ID NO:38;
- and (ii) a light chain comprising an amino acid sequence of SEQ ID NO:37, wherein the anti-TNF antibodies are produced by a method of manufacture comprising:
- a) culturing Chinese Hamster Ovary cells (CHO cells), wherein the CHO cells comprise a nucleic acid encoding the anti-TNF antibodies;
- b) expressing the anti-TNF antibodies in the CHO cells;
- and,
- c) purifying the anti-TNF antibodies,
- wherein the oligosaccharide profile of the anti-TNF antibodies comprises:
- a) total neutral oligosaccharide species >99.0%;
- b) total charged oligosaccharide species <1.0%;
- c) individual neutral oligosaccharide species G0F>60.0%;
- d) individual neutral oligosaccharide species G1F<20.0%;
- or
- e) individual neutral oligosaccharide species G2F<5.0%.
10 more elements
A composition comprising anti-TNF antibodies:
- (i) a heavy chain comprising an amino acid sequence of SEQ ID NO:38;
- and (ii) a light chain comprising an amino acid sequence of SEQ ID NO:37, wherein the anti-TNF antibodies are expressed in Chinese Hamster Ovary cells (CHO cells), wherein the oligosaccharide profile of the anti-TNF antibodies comprises:
- a) total neutral oligosaccharide species >99.0%;
- b) total charged oligosaccharide species <1.0%;
- c) individual neutral oligosaccharide species G0F>60.0%;
- d) individual neutral oligosaccharide species G1F<20.0%;
- or
- e) individual neutral oligosaccharide species G2F<5.0%.
5 more elements
Dependencies are inferred from the claim text.