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US 2025/0019399 A1Published application
Bret-based mpro biosensor with an increased rate of cleavage
AssigneeQatar Foundation for Education, Science and Community Development
InventorsAnupriya M. Geethakumari, Kabir H. Biswas
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Abstract
Example systems, methods, and apparatus are disclosed herein for a BRET-based Miro biosensor including an mNeonGreen (mNG) reporter protein, a NanoLuc (NLuc) reporter protein, and x repeats of an N-terminal autocleavage peptide sequence of Mpro. The x repeats of an N-terminal autocleavage peptide sequence of Mpro are located between the mNG reporter protein and the NLuc reporter protein.
Claims20
A BRET-based Mpro biosensor comprising:
- an mNeonGreen (mNG) reporter protein;
- a NanoLuc (NLuc) reporter protein;
- and
- x repeats of an N-terminal autocleavage peptide sequence of Mpro;
- wherein the x repeats of an N-terminal autocleavage peptide sequence of Mpro are located between the mNG reporter protein and the NLuc reporter protein.
2 more elements
A BRET-based Mpro biosensor comprising:
- an mNeonGreen (mNG) reporter protein;
- a NanoLuc (NLuc) reporter protein;
- x repeats of an N-terminal autocleavage peptide sequence of Mpro;
- a first nanobody;
- and
- a second nanobody;
- wherein the x repeats of an N-terminal autocleavage peptide sequence of Mpro are located between the mNG reporter protein and the NLuc reporter protein.
4 more elements
A method of creating a BRET-based Mpro biosensor comprising:
- placing x repeats of an N-terminal autocleavage peptide sequence of Mpro between an mNeonGreen (mNG) reporter protein and a NanoLuc (NLuc) reporter protein.
Dependencies are inferred from the claim text.