High Efficiency Methods for Isolating Proteins

A method for reducing an environmental impact of industrial scale isolation of a first protein from a blood product, comprising:

• (1) adding a precipitating salt to a volume of the blood product comprising the first protein, a second protein, a third protein, and a fourth protein at ambient temperature to generate a supernatant and a precipitate, wherein the blood product has a volume 2,000 L or more or is derived from 2,000 L or more of blood plasma;
• (2) applying one of the supernatants or the precipitates to a first ion exchange media comprising a first charged group to generate a first flow-through fraction comprising the first protein and the third protein, wherein the second protein is retained on the first chromatography media;
• and
• (3) applying the first flow-through fraction to a second ion exchange media comprising a second charged group to generate a second flow-through fraction comprising the first protein, wherein the first charged group and the second charged group have opposing charges, and wherein chromatography conditions are selected such that both the first protein and the third protein can bind to the second ion exchange media, and
• wherein steps (1) to (3) are performed at ambient temperature and completed in less than 24 hours from initiation of step (1).