Integrated droplet chip

An integrated droplet chip, characterized in that it comprises a chip body (1), the chip body (1) has a reaction chamber (11) and a sample adding chamber (12), and said chip body (1) being constructed with droplet generating structure, oil-liquid interface (31), gas-liquid interface (32) and fluorescence detection area (33), the gas-liquid interface (32) communicates with the reaction chamber (11), and the sample adding chamber (12) communicates with the droplet generating structure, and the oil-liquid interface (31) communicates with the droplet generating structure:

• when the droplets are generated, a first pressure difference is formed between the sample adding chamber (12) and the gas-liquid interface (32), and a second pressure difference is formed between the oil-liquid interface (31) and the gas-liquid interface (32), the first pressure difference and the second pressure difference respectively drive the sample in the sample adding chamber (12) and the generated oil of the oil-liquid interface (31) to enter the droplet generating structure, the generated droplets (4) enter and are stored in the reaction chamber (11);
• during the droplet detection, the external pressure drives the detection pushing oil (5) to enter the reaction chamber (11) from the gas-liquid interface (32), so that the droplets (4) in the reaction chamber (11) flow out of the reaction chamber (11) and enter into the droplet generating structure, and the external pressure drives the detection separation oil to enter the droplet generating structure from the oil-liquid interface (31), and the detection separation oil separates the droplets (4) flowing out of the reaction chamber (11) into the droplet generating structure to form a queue, which enters the fluorescence detection area (33).