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OwnerJatin PANWAR, Ramesh UTHARALA +2inventors
InventorsJatin PANWAR, Ramesh UTHARALA, Christoph MERTEN, Laura FENNELLY
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Disclosed herein is a method of operating a microfluidic system, a microfluidic system for manipulating sample objects, and a control system for operating a microfluidic system. The method according to the invention is for operating a microfluidic system that comprises a manipulation zone for manipulating sample objects flowing through the manipulation zone, a first detection zone arranged upstream of or in the manipulation zone and a second detection zone arranged upstream of, in or downstream of the manipulation zone. A first count is determined that characterizes a number of sample objects flowing through the first detection zone. Sample objects are manipulated in the manipulation zone. A second count is determined that characterizes a number of sample objects flowing through the second detection zone. One or more manipulation parameters for manipulating the sample objects in the manipulation zone are adjusted based on the first count and the second count.
A method (500) of operating a microfluidic system (100, 200, 200′, 300), wherein the microfluidic system (100, 200, 200′, 300) comprises a manipulation zone (116) for manipulating sample objects (102A, 102B) flowing through the manipulation zone (116), a first detection zone (114) arranged upstream of or in the manipulation zone (116) and a second detection zone (118) arranged upstream of, in or downstream of the manipulation zone (116), the method (500) comprising:
The method (500) of any one of the preceding claims, wherein manipulating the sample objects (102A, 102B) in the manipulation zone (116) comprises deflecting the sample objects (102A, 102B) into one of a plurality of sorting channels (106A, 106B) based on a sorting control signal and the second count (N2) characterizes a number of sample objects (102A, 102B) flowing along one of the sorting channels (106A, 106B).
The method (500) of any one of the preceding claims, wherein manipulating the sample objects (102A, 102B) in the manipulation zone (116) comprises merging two or more sample objects (102A, 102B) to a composite sample object.
The method (500) of any one of the preceding claims, wherein manipulating the sample objects (102A, 102B) in the manipulation zone (116) comprises generating a force on the sample objects (102A, 102B) by applying a voltage to an electrode (122) of the microfluidic system (100, 200, 200′, 300) and the one or more manipulation parameters (x1, x2, y1, y2, . . . ) comprise one or more of an amplitude (AV) of the voltage, a frequency (fV) of the voltage, a pulse duration (PV) of the voltage, a pulse delay (LV) of the voltage, and one or more flow rates (QD, QC, QW) of fluid flows in the microfluidic system (100, 200, 200′, 300).
The method (500) of any one of the preceding claims, wherein the method (500) further comprises:
The method (500) of any one of the preceding claims, wherein the sample objects (102A, 102B) are microdroplets forming a dispersed phase in a continuous phase and the method (500) further comprises adjusting one or more of an input flow rate (QD) of the dispersed phase, an input flow rate (QC) of the continuous phase and a withdrawal rate (QW) of the dispersed and continuous phases based on the first count (N1) and the second count (N2).
The method (500) of any one of the preceding claims, further comprising determining one or more of a flow rate, a spacing, a size, and a velocity of the sample objects (102A, 102B) in the first detection zone (114) and/or in the second detection zone (118).
A microfluidic system (100, 200, 200′, 300) for manipulating sample objects (102A, 102B), wherein the microfluidic system (100, 200, 200′, 300) comprises:
A control system (400) for operating a microfluidic system (100, 200, 200′, 300), wherein the microfluidic system (100, 200, 200′, 300) comprises a manipulation zone (116) for manipulating sample objects (102A, 102B) flowing through the manipulation zone (116), a first detection zone (114) arranged upstream of or in the manipulation zone (116) and a second detection zone (118) arranged upstream of, in or downstream of the manipulation zone (116), the control system (400) comprising:
Dependencies are inferred from the claim text.