Method for efficiently infecting human natural killer cells and other immune cells with pseudovirus
AssigneeBeijing Gene MINK Biotechnology CO.LTD
InventorsYongfeng Liu, Zhihua Lu
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Abstract
The present disclosure belongs to the field of biotechnology, and specifically relates to a method for efficiently infecting human natural killer (NK) cells and other immune cells with a pseudovirus. Specifically, a viral transfection system provided in the present disclosure has an envelope plasmid with a protein having an X—Y—Z structure. The X is an extracellular (ex) structure of a gibbon ape leukemia virus (GALV) envelope glycoprotein, the Y is a transmembrane (TM) structure of the GALV envelope glycoprotein, and the Z is an intracellular segment portion of a murine virus gene.
Claims11
A method for preparing natural killer (NK) cells expressing a target gene, comprising the steps of transfecting cells with a vector composition containing an envelope plasmid, collecting a viral liquid, and contacting the viral liquid with NK cells,
- the envelope plasmid containing a fusion protein having an X—Y—Z structure, the X having an amino acid sequence as shown in SEQ ID NO.1, the Y having an amino acid sequence as shown in SEQ ID NO.2, and the Z having an amino acid sequence as shown in SEQ ID NO.3.
An application of an envelope plasmid or a composition thereof in preparing NK cells expressing a target gene,
- an envelope plasmid containing a fusion protein having an X—Y—Z structure, the X having an amino acid sequence as shown in SEQ ID NO.1, the Y having an amino acid sequence as shown in SEQ ID NO.2, and the Z having an amino acid sequence as shown in SEQ ID NO.3.
Dependencies are inferred from the claim text.