Human inducibility controllable t-cell and method for preparing same
AssigneeRegCell Co., Ltd.
InventorsNorihisa Mikami, Shimon Sakaguchi
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Abstract
According to the present disclosure, there is provided an inducible regulatory T cell having high functionality and a stable immunosuppressive function. The present disclosure provides an inducible regulatory human T cell having at least one feature selected from the group consisting of FoxP3 positivity, CTLA4 positivity, NT5E positivity, ITGAE (CD103) positivity, AREG positivity, CD172g positivity, and CD26 positivity.
Claims38
An inducible regulatory human T cell having at least one feature selected from the group consisting of FoxP3 positivity, CTLA4 positivity, NT5E positivity, ITGAE (CD103) positivity, AREG positivity, CD172g positivity, and CD26 positivity.
An inducible regulatory human T cell which is NT5E-positive.
An inducible regulatory human T cell which is ITGAE (CD103)-positive.
An inducible regulatory human T cell which is AREG-positive.
An inducible regulatory human T cell which is CD172g-positive.
An inducible regulatory human T cell which is CD26-positive.
An inducible regulatory human T cell which is CTLA4-positive.
An inducible regulatory human T cell obtained by a method including the steps of:
- (a) stimulating a CD4-positive T cell or a CD8-positive T cell in human peripheral blood in a first basal medium for about 1 to about 5 days;
- (b) dormant culturing the cell obtained in step (a) in a medium containing IL-2 for at least about 1 to about 3 days;
- (c) stimulating the cell obtained in step (b) in a second basal medium for about 1 to about 5 days;
- and
- (d) dormant culturing the cell obtained in step (c) in a medium containing IL-2 for at least about 1 to about 3 days.
A cell population comprising an inducible regulatory human T cell, wherein
- the cell population has a proportion of NT5E positivity of about 50% or more.
A cell population comprising an inducible regulatory human T cell, wherein
- the cell population has a proportion of ITGAE (CD103) positivity of about 50% or more.
A cell population comprising an inducible regulatory human T cell, wherein
- the cell population has a proportion of AREG positivity of about 50% or more.
A cell population comprising an inducible regulatory human T cell, wherein
- the cell population has a proportion of CD172g positivity of about 50% or more.
A cell population comprising an inducible regulatory human T cell, wherein
- the cell population has a proportion of CD26 positivity of about 50% or more.
A cell population comprising an inducible regulatory human T cell, wherein
- the cell population has a proportion of CTLA4 positivity of about 50% or more.
A method for preparing an inducible regulatory human T cell, the method comprising the steps of:
- (a) stimulating a CD4-positive T cell or a CD8-positive T cell in human peripheral blood in a first basal medium for about 1 to about 5 days;
- (b) dormant culturing the cell obtained in step (a) in a medium containing IL-2 for at least about 1 to about 3 days;
- (c) stimulating the cell obtained in step (b) in a second basal medium for about 1 to about 5 days;
- and
- (d) dormant culturing the cell obtained in step (c) in a medium containing IL-2 for at least about 1 to about 3 days.
Dependencies are inferred from the claim text.