Igg4 hinge-containing chimeric antigen receptors targeting glypican-3 (gpc3) and use thereof
AssigneeThe U.S.A., as represented by the Secretary, Department of Health and Human Services
InventorsMitchell Ho, Aarti Kolluri, Nan Li
Life of this patent
Estimating the term…
Abstract
Optimized chimeric antigen receptors (CARs) targeting glypican-3 (GPC3) and having a 12-amino acid hinge region derived from human IgG4 are described. The optimized CARs also include a transmembrane domain from either CD8 or CD28, an intracellular co-stimulatory domain and an intracellular signaling domain. Immune cells or induced pluripotent stem cells expressing the optimized CARs can be used to treat GPC3-positive solid tumors.
Claims30
A chimeric antigen receptor (CAR), comprising:
- an extracellular antigen-binding domain that specifically binds glypican-3 (GPC3);
- a hinge region consisting of the IgG4 hinge region set forth as SEQ ID NO:
- 43 or SEQ ID NO:
- 52;
- a transmembrane domain;
- an intracellular co-stimulatory domain;
- and
- an intracellular signaling domain.
7-8. (canceled)
10-11. (canceled)
14-15. (canceled)
(canceled)
(canceled)
Dependencies are inferred from the claim text.