Compositions for cell-based therapies and related methods
AssigneeSigilon Therapeutics, Inc.
InventorsLauren Emily Barney, Hozefa Bandukwala, Susan J. Drapeau, Lauren E. Jansen
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Abstract
Described herein are hydrogel capsule compositions which comprise a population of hydrogel capsules and a pharmaceutically acceptable solution. The hydrogel capsules in the population comprise a hydrogel-forming polymer and encapsulate a plurality of cells.
Claims50
A composition comprising a population of hydrogel capsules disposed in a pharmaceutically acceptable aqueous solution, wherein each hydrogel capsule in the population encapsulates a plurality of live mammalian cells, wherein the solution has a pH of between 6.0 and 9.0 at 12° C. to 30° C. (e.g., about 15-25° C.), and comprises a calcium salt at an elemental calcium concentration of between about 1.0 millimolar (mM) and about 10 mM.
A sealed container comprising the composition of any one of the above claims.
A method of making a sealed container comprising a hydrogel capsule composition, wherein the method comprises:
- (i) providing a population of hydrogel capsules encapsulating live mammalian cells, optionally wherein the mammalian cells are genetically modified to express and secrete a therapeutic substance, e.g., a therapeutic polypeptide;
- (ii) combining the population of hydrogel capsules with a pharmaceutically acceptable aqueous solution;
- (iii) placing a desired volume of the capsule composition into a biocompatible, sealable container in a manner that produces a capsule layer in which substantially all of the capsules in the composition volume are distributed substantially uniformly across the bottom of the container at a depth equal to about 1.00 to about 1.25 times the mean diameter of the capsules in the composition;
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- (iv) sealing the container.
Dependencies are inferred from the claim text.