Pharmaceutical Preparation for Topical Delivery of Botulinum Toxin

A method of treatment comprising,

• a. topically administering a nano-emulsion pharmaceutical composition to a subject, comprising one or more proteins derived from Clostridium botulinum;
• b. wherein the one or more proteins comprise one or more from botulinum toxin A (BoNT/A), BoNT/B, BoNT/C, BoNT/D, BoNT/E. BoNT/F, or BoNT/G, or their different forms, or any combination thereof;
• and
• c. wherein the pharmaceutical composition effectively treats local and systemic conditions comprising:
• neuromuscular, gastrointestinal, diabetic, cardiovascular, reproductive issues, skin problems;
• and/or to work as a local aesthetic.

A method of preparing an encapsulation of a toxin or complex, comprising the steps:

• (1) preparing a solution A by mixing sodium hyaluronate, propylene glycol, Tween-80 and saponin in water by continuous stirring;
• (2) preparing a solution B by mixing phenoxy ethanol and, carylic acid or capric triglyceride, together;
• (3) mixing solution B into solution A by continuous stirring for 15 minutes to form solution C;
• (4) mixing Botulinum toxin and 10 mM Sodium Phosphate buffer to a pH of 7.1, and adding to solution C;
• and
• (5) stirring solution C at room temperature continuously for 15-20 minutes.