Non-human animals comprising a modified cacng1 locus
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsTrevor Stitt, Susannah Brydges, Alexander O. Mujica, Roxanne Ally
Life of this patent
Estimating the term…
Abstract
Non-human animal cells and non-human animals comprising a humanized Cacng1 locus and methods of using such non-human animal cells and non-human animals are provided. Non-human animal cells or non-human animals comprising a humanized Cacng1 locus express a human CACNG1 protein or fragments thereof.
Claims104
A non-human animal cell, wherein the non-human animal cell comprises a nucleic acid sequence encoding a heterologous Calcium Voltage-Gated Channel Auxiliary Subunit Gamma 1 (CACNG1) protein or a portion thereof.
The non-human animal cell of any one of claims 1-8, wherein the heterologous CACNG1 protein or the portion thereof comprises an amino acid sequence of a human CACNG1 protein or a portion thereof.
The non-human animal cell of any one of claims 1-21, wherein the non-human animal cell is a mouse cell.
A non-human animal comprising a nucleic acid sequence encoding a heterologous Calcium Voltage-Gated Channel Auxiliary Subunit Gamma 1 (CACNG1) protein or a portion thereof.
A non-human animal genome, wherein the non-human animal genome comprises a nucleic acid sequence encoding a heterologous Calcium Voltage-Gated Channel Auxiliary Subunit Gamma 1 (CACNG1) protein or a portion thereof.
A chimeric nucleic acid molecule, comprising a nucleic acid sequence of a non-human animal Cacng1 gene that (a) encodes a CACNG1 protein and (b) is modified to comprise a replacement of a sequence encoding the CACNG1 protein or portion thereof with a homologous sequence encoding a heterologous CACNG1 protein or a portion thereof,
- wherein the chimeric nucleic acid molecule encodes a functional CACNG1 protein.
A non-human animal, non-human animal cell, or non-human animal genome comprising a knockout mutation of an endogenous Cacng1 gene.
A method of making a CACNG1 knockout non-human animal comprising modifying an endogenous Cacng1 locus of the non-human animal to comprise a knockout mutation.
A targeting vector comprising:
- (i) a 5′ homology arm;
- and
- (ii) a 3′ homology arm,
- wherein the 5′ homology arm and 3′ homology arm undergo homologous recombination with a non-human animal Cacng1 locus of interest, and
- wherein following homologous recombination with the non-human animal Cacng1 locus of interest, the targeting vector inserts a knockout mutation in the non-human animal Cacng1 gene at the non-human animal Cacng1 locus of interest.
A non-human animal, non-human animal cell, or non-human animal genome made according to any method described herein.
Dependencies are inferred from the claim text.