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US 2025/0011883 A1Published application
Polymerase Chain Reaction Primers and Probes for Mycobacterium Tuberculosis
AssigneeRutgers, The State University of New Jersey
InventorsDavid Alland, Soumitesh Chakravorty
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Abstract
The present invention relates to novel primers and sloppy molecular beacon and molecular beacon probes for amplifying segments from different genes in Mycobacterium tuberculosis for identifying the presence of M.tb DNA and/or resistance to anti-tuberculosis drugs.
Claims7
A method for detecting drug resistance in M. tuberculosis, comprising
- amplifying a first nucleic acid target sequence with a first primer pair to generate a first amplicon, the first primer pair being specific for a portion of a first region of a katG gene and each primer having a sequence that is at least 85% identical to an oligonucleotide sequence selected from the group consisting of SEQ ID Nos:
- 7-11, and
- detecting a first mutation in the first amplicon,
- wherein the presence of the first mutation is indicative of the drug resistance.
1 more element
Dependencies are inferred from the claim text.