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US 2025/0011767 A1Published application
Compositions and methods for production of circular nucleic acid molecules
AssigneeCircularis Biotechnologies, Inc.
InventorsPaul A. Feldstein, Matthew Falkowski, Christen Yuen
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Abstract
In certain aspects, the present disclosure provides nucleic acid molecules containing ribozyme catalytic cores for production of circularized RNA containing a nucleic acid sequence of interest, as well as methods of preparation and methods of use.
Claims81
A nucleic acid molecule comprising, in 5′ to 3′ order:
- (i) an upstream ribozyme catalytic core,
- (ii) an upstream cleavage site,
- (iii) a central ribozyme catalytic core,
- (iv) a downstream cleavage site, and
- (v) a downstream ribozyme catalytic core;
- wherein the nucleic acid molecule further comprises a sequence of interest between (ii) and (iv),
- wherein the upstream ribozyme catalytic core is configured to cleave the upstream cleavage site to produce an upstream cleaved terminus and the downstream ribozyme catalytic core is configured to cleave the downstream cleavage site to produce a downstream cleaved terminus, and
- wherein the central ribozyme catalytic core is configured to join the upstream cleaved terminus and the downstream cleaved terminus to produce a circular nucleic acid molecule comprising the sequence of interest.
5 more elements
A nucleic acid molecule comprising, in 5′ to 3′ order:
- (i) an upstream ribozyme catalytic core,
- (ii) an upstream cleavage site,
- (iii) a central ribozyme catalytic core, and
- (iv) a downstream cleavage site,
- wherein the nucleic acid molecule further comprises a sequence of interest between (ii) and (iv),
- wherein the upstream ribozyme catalytic core is configured to cleave the upstream cleavage site to produce an upstream cleaved terminus and the central ribozyme catalytic core is configured to cleave the downstream cleavage site to produce a downstream cleaved terminus, and
- wherein the central ribozyme catalytic core is configured to join the upstream cleaved terminus and the downstream cleaved terminus to produce a circular nucleic acid molecule comprising the sequence of interest.
4 more elements
A nucleic acid molecule comprising, in 5′ to 3′ order:
- (i) an upstream cleavage site,
- (ii) a central ribozyme catalytic core,
- (iii) a downstream cleavage site, and
- (iv) a downstream ribozyme catalytic core,
- wherein the nucleic acid molecule further comprises a sequence of interest between (i) and (iii),
- wherein the central ribozyme catalytic core is configured to cleave the upstream cleavage site to produce an upstream cleaved terminus and the downstream ribozyme catalytic core is configured to cleave the downstream cleavage site to produce a downstream cleaved terminus, and
- wherein the central ribozyme catalytic core is configured to join the upstream cleaved terminus and the downstream cleaved terminus to produce a circular nucleic acid molecule comprising the sequence of interest.
4 more elements
Dependencies are inferred from the claim text.