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US 2025/0011754 A1Published application
Rna target enrichment or depletion of biological samples
AssigneeAltratech Limited
InventorsBrian O'Farrell, Claire O'Connell, Kaylyn Oshaben, Daniel Appella
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Abstract
A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.
Claims19
A method of target enrichment in a sample, the method comprising the steps of:
- introducing a probe comprising a left-handed PNA molecule linked to a capture moiety into a sample comprising target analyte;
- capturing, with the capture moiety, the target analyte;
- and
- binding the left-handed PNA molecule with a complementary PNA molecule comprising a left-handed chiral structure, the complementary PNA molecule being attached to a surface to thereby enrich for target analyte.
1 more element
Dependencies are inferred from the claim text.