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US 2025/0011749 A1Published application
Enzymatic compositions for carbohydrate antigen cleavage, methods, uses, apparatuses and systems associated therewith
AssigneeThe University of British Columbia
InventorsStephen G. WITHERS, Peter RAHFELD, Jayachandran KIZHAKKEDATHU
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Abstract
Provided herein are enzymatic compositions for carbohydrate antigen cleavage, methods, uses, apparatuses and sys-O tems associated therewith. In particular, the composition comprises two enzymes, GalNAcDeacetylase and Galactosaminidase and the el composition may further comprise a crowding agent. Furthermore, the compositions described herein were found to have activity a C) temperatures and pH levels suitable for cell viability.
Claims12
-22. (canceled)
A method for enzymatically cleaving A-antigens from erythrocytes, the method comprising:
- (a) combining a GalNAcDeacetylase protein and a Galactosaminidase protein with (i) blood comprising type A antigen;
- or (ii) erythrocytes of A type or AB type;
- (b) incubating the enzymes with the (i) the blood;
- or (ii) the erythrocytes of an A type or AB type;
- for a period of time sufficient to allow the enzymes to cleave A-antigens from the blood or erythrocytes,
- wherein the GalNAcDeacetylase protein, the Galactosaminidase protein, or both the GalNAcDeacetylase protein and the Galactosaminidase protein is derived from Flavonifractor plautii.
3 more elements
(canceled)
-43. (canceled)
Dependencies are inferred from the claim text.