Production of viruses in cell culture
AssigneesCommonwealth Scientific and Industrial Research Organisation, University of Georgia Research Foundation, Inc.
InventorsAndrew Bean, John William Lowenthal, Luis Fernando Malaver-Ortega, Ralph A. Tripp
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Abstract
The present invention relates to methods of replicating viruses in vitro. In particular, the invention relates to a genetically modified population of cells, and/or a population of cells treated with an exogenous compound, wherein the cells are capable of producing more virus than cells lacking the genetic modification and/or lacking treatment with the exogenous compound. The invention also relates to methods of producing populations of such cells, as well as the use of the viruses obtained to prepare vaccine compositions.
Claims26
A method of replicating a Paramyxoviridae virus, the method comprising
- 1) obtaining a population of cells having a genetic modification introduced by a programmable nuclease which reduces the expression of Interleukin 1 receptor type 1 (IL-1RA) in the cells when compared to isogenic cells lacking the genetic modification,
- 2) inoculating the cells in vitro with the virus, and
- 3) culturing the cells for a predetermined period of time to replicate the virus.
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A population of cells in vitro comprising a Paramyxoviridae virus and a genetic modification introduced by a programmable nuclease which reduces the expression of Interleukin 1 receptor type 1 (IL-1RA) in the cells when compared to isogenic cells lacking the genetic modification.
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Dependencies are inferred from the claim text.