Methods and formulations for denaturing dna
AssigneeIllumina, Inc.
InventorsDale Weekes, Oliver Miller
Life of this patent
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Abstract
Disclosed herein are formulations and methods for denaturing DNA that utilize betaine as the denaturant. In some examples, the formulations include additives other than betaine, such as, for example, dimethyl sulfoxide (DMSO) and/or diethylene glycol (DEG).
Claims30
A solution formulation for denaturing double-stranded DNA (dsDNA), the solution formulation comprising:
- betaine;
- and
- at least one organic solvent.
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A method of denaturing double-stranded DNA (dsDNA), the method comprising:
- contacting the dsDNA in a fluid-filled container with a denaturant comprising betaine.
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A kit for denaturing double-stranded DNA (dsDNA), the kit comprising:
- a denaturant comprising betaine and diethylene glycol (DEG) in a solution;
- and
- a buffer.
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Dependencies are inferred from the claim text.