Recombinant manufacture of c-20 terpenoid alcohols

A method for the manufacture of at least one C-20 terpenoid alcohol comprising the steps of:

• a) converting geranylgeranyl pyrophosphate into copalyl diphosphate (CPP) or labda-13-en-8-ol diphosphate (LPP);
• and
• b) converting CPP or LPP into at least one C-20 terpenoid alcohol, wherein said conversion is carried out by a polypeptide exhibiting diterpene alcohol synthase activity, wherein said diterpene alcohol synthase activity is capable of converting CPP into manool, LPP into sclareol and/or LPP into abienol,
• wherein said polypeptide comprises an amino acid sequence selected from the group consisting of:
• i) an amino acid sequence as shown in SEQ ID NO:
• 3, 4, 5, 6, 7, 8, 9, 10, or 34;
• ii) an amino acid sequence which is at least 60% identical to the amino acid sequence of SEQ ID NO:
• 3, 4, 5, 6, 7, 8, 9, 10, or 34;
• iii) an amino acid sequence encoded by a nucleic acid sequence as shown in SEQ ID NO:
• 1, 2, 16, 17, 18, or 35;
• iv) an amino acid sequence encoded by a nucleic acid sequence which is at least 60% identical to the nucleic acid sequence as shown in SEQ ID NO:
• 1, 2, 16, 17, 18, or 35;
• and
• v) a fragment of the amino acid sequence of (i), (ii), (iii), or (iv), said fragment encoding a polypeptide exhibiting a diterpene alcohol synthase activity capable of converting CPP into manool, LPP into sclareol and/or LPP into abienol.

A polypeptide exhibiting diterpene alcohol synthase activity, wherein said diterpene alcohol synthase activity is capable of converting copalyl diphosphate (CPP) into manool, labda-13-en-8-ol diphosphate (LPP) into sclareol and/or LPP into abienol, said polypeptide having an amino acid sequence selected from the group consisting of:

• a) an amino acid sequence as shown in SEQ ID NO:
• 3, 4, 5, 6, 7, 8, 9, 10, or 34;
• b) an amino acid sequence which is at least 60% identical to the amino acid sequences as shown in SEQ ID NO:
• 3, 4, 5, 6, 7, 8, 9, 10, or 34;
• c) an amino acid sequence encoded by a nucleic acid sequence as shown in SEQ ID NO:
• 1, 2, 16, 17, 18, or 35;
• d) an amino acid sequence encoded by a nucleic acid sequence which is at least 60% identical to the nucleic acid sequence as shown in SEQ ID NO:
• 1, 2, 16, 17, 18, or 35;
• and
• e) a fragment of the amino acid sequence of (a), (b), (c), or (d), said fragment encoding a polypeptide exhibiting a diterpene alcohol synthase activity capable of converting CPP into manool, LPP into sclareol and/or LPP into abienol.