Method for Cell-Free Production of Unspecific Peroxygenases

A method for the production of unspecific peroxygenases (UPO) in cell-free systems using eukaryotic cell extracts, comprising the following steps:

• i) preparing eukaryotic cell extracts for use in cell-free protein synthesis;
• ii) providing a nucleic acid template that contains the genetic information for the UPO;
• iii) providing further components necessary for the translation of proteins, in particular amino acids, energy-rich triphosphates, salts, RNA polymerase;
• and
• iv) combining the cell extract from i), the nucleic acid template from ii) and the other components from iii).