Location-matched growth media formulations for the development of brain tumor organoids
OwnerMilagros Melanie Suarez Palacios, Yuchen Du +1inventors
InventorsMilagros Melanie Suarez Palacios, Yuchen Du, Xiaonan Li
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Abstract
Disclosed are formulations and methods for developing patient-derived brain cancer organoids, including site-specific patient-derived orthotopic xenograft (PDOX) organoids.
Claims17
A forebrain region tumor culture formulation comprising a basal medium supplemented with recombinant human basic fibroblast growth factor (FGF), recombinant human epidermal growth factor (EGF), brain-derived neurotrophic factor (BDNF), glial cell-derived neurotrophic factor (GDNF), neurotrophin 3 (NT-3), ciliary neurotrophic factor (CNTF), and wingless-relate integration site 3A (WNT-3A).
A midbrain region tumor culture formulation comprising a basal medium supplemented with recombinant FGF-8 and recombinant sonic hedgehog (SHH).
A hindbrain region cancer cell culture formulation comprising a basal medium supplemented with recombinant FGF, recombinant FGF-19, and recombinant stromal cell-derived factor 1 alpha (SDF-1-α).
A hindbrain region cancer cell culture formulation comprising a basal medium supplemented with recombinant human basic recombinant FGF, recombinant human EGF, BDNF, GDNF, and NT-3.
Dependencies are inferred from the claim text.