Mutant brd4 polypeptides and methods of use thereof
OwnerAli Shilatifard, Lu Wang +1inventors
InventorsAli Shilatifard, Lu Wang, Bin Zheng
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Abstract
The present invention provides engineered BRD4 polypeptides comprising deletions of one or more of bromodomain 1 (BD1), bromodomain 2 (BD2), and extra terminal domain (ET). Also provided are polynucleotides encoding the engineered BRD4 polypeptides, and constructs and vectors comprising the polynucleotides. Also provided are methods for using the engineered BRD4 polypeptides to assess the effects of candidate BRD4 C-terminal specific inhibitors on Pol II pausing.
Claims20
An engineered bromodomain-containing protein 4 (BRD4) polypeptide comprising a deletion of at least one of bromodomain 1 (BD1), bromodomain 2 (BD2), and extra terminal domain (ET).
An engineered bromodomain-containing protein 4 (BRD4) polypeptide comprising a deletion of a C-terminal fragment of SEQ ID NO:
- 1 or SEQ ID NO:
- 2.
Dependencies are inferred from the claim text.