A method for production of self-replicating, nucleic acid-loaded, virus-like particles (vlp-na) and the uses thereof
AssigneeArizona Board of Regents on behalf of Arizona State University
InventorsQiang Chen, Haiyan Sun, Collin Jugler, Huafang Lai
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Abstract
The present invention provides nucleic acid-loaded virus-like particles (VLPs) that can be produced in plants and are designed to deliver a nucleic acid into mammalian cells. Also provided are pharmaceutical compositions comprising the nucleic acid-loaded VLPs, and methods of making and using the nucleic acid-loaded VLPs.
Claims30
A virus-like particle (VLP) comprising an icosahedral mammalian virus capsid protein and containing a replicon comprising a mammalian promoter operably linked to a heterologous polynucleotide, wherein the 5′ and 3′ ends of the replicon consist of a geminiviral long intergenic region (LIR) sequence.
A method for producing a nucleic acid-loaded VLP, the method comprising:
- a) introducing into a plant cell a first construct comprising a first replicon that comprises a plant promoter operably linked to a first polynucleotide encoding an icosahedral mammalian virus capsid protein, wherein the 3′ end of the first polynucleotide is flanked by a geminiviral SIR sequence, and wherein the 5′ and 3′ ends of the first replicon consist of a geminiviral LIR sequence;
- b) introducing into the plant cell a second construct comprising a second replicon that comprises a mammalian promoter operably linked to a second, heterologous polynucleotide, wherein the 3′ end of the heterologous polynucleotide is flanked by a geminiviral SIR sequence, and wherein the 5′ and 3′ ends of the second replicon consist of a geminiviral LIR sequence;
- c) introducing a third construct comprising a plant promoter operably linked to a third polynucleotide encoding Rep and RepA;
- and
- d) harvesting the VLPs produced by the plant cell.
Dependencies are inferred from the claim text.