Thermally responsive partitions for devices and systems and methods of using same

A testing system comprising:

• a test assembly, the test assembly comprising an inlet configured to accept a sample comprising an analyte, a plurality of regions, and at least one solid aliphatic partition disposed therein, wherein adjacent regions of the plurality of regions are separated by one of the at least one solid aliphatic partition, and the plurality of regions includes a binding region and a detecting region, and the at least one solid aliphatic partition has a melting point of 40° C. to 65° C.;
• wherein the binding region comprises a plurality of magnetic beads having a plurality of first capture molecules attached thereto, wherein the first capture molecules have a specific affinity for the analyte and a plurality of second capture molecules having a detectable label attached thereto, wherein the second capture molecules have a specific affinity for the analyte,
• wherein the detecting region comprises a detection composition configured to enable spectrophotometric measurement of the composition;
• and
• a magnet configured to apply a magnetic field along a sequential path, whereby the plurality of magnetic beads move sequentially along the sequential path upon application of a magnetic force from the magnet, wherein the sequential path includes the binding region and the detecting region.