Sample carrier and method for imaging a sample

A method for imaging a sample with an imaging system, the method comprising the following steps:

• providing a sample carrier having an optical medium for embedding the sample, the optical medium having a first refractive index, and a window portion defining two parallel surfaces, comprising an optically transparent material that has a second refractive index, and is arranged at a bottom side of the sample carrier, wherein the first and second refractive indices do not deviate by more than 2.5%;
• arranging the sample in the sample carrier by immersing the sample in the optical medium;
• positioning the sample carrier in a sample carrier receiving portion of the imaging device;
• and
• capturing at least one image of the sample with an optical detection system of the imaging device, wherein the optical detection system comprises at least one objective arranged below the sample carrier and configured to receive detection light emitted by the sample, and wherein the optical axis of the at least one objective and the parallel surfaces of the window portion enclose an oblique angle.