US 2024/0404303 A1 · SYSTEMS AND METHODS FOR LABEL-FREE MULTI-HISTOCHEMICAL VIRTUAL STAINING

Abstract

Systems and methods for multi-spectral and label-free autofluoresence histochemical instant virtual staining are provided. The system includes a sample holder, one or more light sources, an imaging device, and a processor means. The sample holder is configured to secure a sample on a movable structure that is movable in at least two dimensions. The one or more light sources are configured to obliquely illuminate the sample for excitation of the sample. The imaging device is configured to capture images of the sample and the processing means is configured to receive the images of the sample and process the images in accordance with a histochemical virtual staining process. The images include an autofluorescence image and the histochemical virtual staining process includes subdividing the autofluorescence image into a plurality of regions, global sampling a selected region of the autofluorescence image, and classifying the autofluorescence image as one of a plurality of real image classifications or a fake image classification.

Claims14

Dependent claims

A method for label-free autofluorescence histochemical instant virtual staining comprising:

subdividing a pair of images of a sample into a plurality of regions, wherein one of the pair of images comprises a first autofluorescence image and a first corresponding image;
selecting one of the subdivided regions of each of the pair of images;
global sampling the selected region of each of the pair of images;
local sampling of portions of the selected region of each of the pair of images, each portion of the selected region of each of the pair of images comprising a multi-pixel cropped patch;
encoding and decoding the locally sampled cropped patch of each of the pair of images to generate a second autofluorescence image and a second corresponding image;
and
classifying the second autofluorescence image and the second corresponding image as one of a plurality of real image classifications or a fake image classification, wherein global sampling of the selected region of each of the pair of images comprises determining a probability of the selected region being trained in the current iteration in response to a ratio of a similarity index of the selected region to similarity indexes of unselected regions.

4 more elements

A system for label-free histochemical virtual staining comprising:

a sample holder configured to secure a sample on a movable structure, the movable structure movable in at least two dimensions;
one or more light sources configured to obliquely illuminate the sample for excitation thereof;
an imaging device configured to capture images of the sample;
and
a processing means configured to receive the images of the sample and process the images in accordance with a histochemical virtual staining process, wherein the images comprise an autofluorescence image, and wherein the histochemical virtual staining process comprises subdividing the autofluorescence image into a plurality of regions, global sampling a selected region of the autofluorescence image, and classifying the autofluorescence image as one of a plurality of real image classifications or a fake image classification.

2 more elements

Dependencies are inferred from the claim text.

Systems and methods for label-free multi-histochemical virtual staining

Life of this patent

Systems and methods for label-free multi-histochemical virtual staining

Life of this patent

Abstract

Claims14