System and method of evaluating fluidic substance

A method of evaluating a fluidic substance having particles, the method comprising:

• capturing, via an image capture device, a reference image of at least a portion of a container that is in an empty state;
• determining, via a processor, a reference grayscale value of at least a portion of the reference image;
• aspirating an aspirated volume of the fluidic substance into the container;
• capturing, via the image capture device, a test image of the container containing the aspirated volume of the fluidic substance;
• determining, via the processor, a test grayscale value of at least a portion of the test image;
• calibrating, via the processor, a universal calibration curve relating particle concentration and grayscale value based on the reference grayscale value and the test grayscale value to generate a calibrated universal calibration curve;
• and
• determining, via the processor, a particle concentration in the fluidic substance based on the test grayscale value and the calibrated universal calibration curve.