System, disease model and methods of using the same
OwnerMark Justin MONDRINOS, Elisabet OLSENinventors
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Abstract
Disclosed are designs that enable rapid production of fully vascularized milliscale explant tissues that will enable preservation and expansion of this precious clinical resource and simultaneous investigation of potential biological underpinnings of diseases. Models include triple negative breast cancer or aggressive lung cancer and quiescent lung interstitium integrated in a robust, easily adaptable millifluidic device. Devices will allow any laboratory that use cultures and/or organoids to construct complex multi-tissue/organ systems. Implementation of these devices can model multiple organ pathologies induced by tumor-derived factors.
Claims27
A composition comprising:
- (a) a solid substrate comprising a first compartment and a second compartment;
- the first and second compartments in fluid communication with each other;
- (b) a plurality of cancer cells;
- (c) a plurality of endothelial cells;
- and
- wherein the cancer cells and the endothelial cells are in separate compartments in fluid communication;
- and
- wherein the composition is membrane-free.
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The method of claim 28 further comprising a step of (b) monitoring the cells for morphologic changes or changes of expression profile of cells after step (a).
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Dependencies are inferred from the claim text.