Methods and systems for determining suitability of compositions for inhibiting growth of polymicrobial samples

A method for providing the therapeutic solution to treat a polymicrobial infection in a patient in need thereof, wherein the patient either has a polymicrobial infection or is suspected of having polymicrobial infection, said method comprises:

• a) obtaining or having obtained a sample from a source of the polymicrobial infection or suspected polymicrobial infection in the patient;
• b) subjecting or having subjected a first portion of the sample to genetic identification testing, wherein genetic identification testing detects and identifies one or more organisms in the sample;
• wherein if genetic identification testing detects one or more organisms in the sample then the patient has a polymicrobial infection;
• c) subsequently subjecting or having subjected a second portion of the sample to genetic resistance marker testing, wherein genetic resistance marker testing is effective for detecting and identifying one or more resistance genes that confers resistance to one or more therapeutic agents;
• d) subjecting or having subjected a third portion of the sample to a fluorescent-based pooled phenotypic antibiotic resistance testing, wherein pooled phenotypic antibiotic resistance testing either or both:
• (i) identifies one or more therapeutic agents to which the polymicrobial infection is resistant, and or (ii) identifies one or more therapeutic agents to which the polymicrobial infection is susceptible,
• e) applying results from (b), (c), and (d) to a predetermined set of thresholds in a database that indicates therapeutic agents that are effective for treating polymicrobial infections, wherein applying results from (b), (c), and (d) identifies at least one therapeutic agent that is effective for treating the polymicrobial infection in the patient;
• and
• f) providing the therapeutic solution to a medical professional to determine treatment for the patient.