Scalable process for on-site biopolymer production for enhanced oil recovery projects

A comprehensive, scalable process for on-site biopolymer production related to enhanced oil recovery projects, containing the following steps of:

• a) bacterium/fungus activation with selected substrates by placing them in a sterile, solid culture medium and incubating at a temperature until reaching a desired polysaccharide production level;
• b) performing a replication sequence in a sterile, liquid culture medium to increase the amount of biomass to obtain a laboratory inoculum c) fermenting in fermentation vats or bioreactors, where substrates are transformed by microorganisms into biopolymers and biomass;
• d) filtration or centrifugation, which causes the biopolymer to separate from the biomass;
• e) a liquid polymer obtained during step d) is mixed with injection water in a static mixer, and then injected directly into a reservoir through an injection well.