US 2024/0401097 A1 · METHOD FOR PREPARING STARCH USING CARBON DIOXIDE, RECOMBINANT MICROORGANISM, AND METHOD FOR CONSTRUCTING RECOMBINANT MICROORGANISM

Abstract

Provided are a method for preparing starch using carbon dioxide, a recombinant microorganism, a method for constructing the recombinant microorganism, and a reagent. The method for preparing starch using carbon dioxide comprises: (1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy; and (2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells. In this way, by utilizing non-optical energy, such as electric energy or hydrogen energy, starch can be effectively prepared inside the microbial cells by fixing carbon dioxide.

Claims45

Dependent claims

A method for preparing starch using carbon dioxide, characterized by comprising:

(1) providing energy and carbon sources for microbial cells on the basis of carbon dioxide and extracellular non-optical energy;
and
(2) generating starch within the microbial cells on the basis of at least one of up-regulated glucose-1-phosphate adenylyltransferase and starch synthase in the microbial cells,
optionally, the extracellular non-optical energy comprises at least one of hydrogen energy and electric energy;
preferably, the metabolism of starch is blocked by at least one of glucan 1,4-α-glucosidase and glycogen phosphorylase in a microorganism.

2 more elements

A recombinant microorganism for preparing starch using carbon dioxide, characterized in that the recombinant microorganism has:

(1) an enzyme system for obtaining energy and carbon sources on the basis of carbon dioxide and extracellular non-optical energy;
and
(2) at least one of up-regulated starch synthase and glucose-1-phosphate adenylyltransferase compared with a wild type of the microorganism,
optionally, the extracellular non-optical energy comprises at least one of hydrogen energy and electric energy;
preferably, the recombinant microorganism has at least one of down-regulated glucan 1,4-α-glucosidase and glycogen phosphorylase compared with the wild type of the microorganism.

2 more elements

Dependencies are inferred from the claim text.

Method for preparing starch using carbon dioxide, recombinant microorganism, and method for constructing recombinant microorganism

Life of this patent

Method for preparing starch using carbon dioxide, recombinant microorganism, and method for constructing recombinant microorganism

Life of this patent

Abstract

Claims45