Methods for preventing inter-droplet transfer of a fluorescent product in aqueous droplets by forming a soluble complex with cyclodextrin
AssigneePattern Bioscience, Inc.
InventorRoss JOHNSON
Life of this patent
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Abstract
A method for retaining fluorescent molecules within aqueous droplets suspended in an emulsion includes combining a plurality of living cells, a plurality of fluorescent molecules, and a plurality of cyclodextrin molecules in an aqueous solution. The method also includes emulsifying the aqueous solution with a hydrophobic fluid to form the aqueous droplets. At least one of the aqueous droplets includes (i) a single living cell or a single cell species in a homogenous aggregate of the plurality of living cells, (ii) at least one fluorescent molecule of the plurality of fluorescent molecules, and (iii) at least one cyclodextrin molecule of the plurality of cyclodextrin molecules.
Claims27
A method for retaining fluorescent molecules within aqueous droplets suspended in an emulsion, the method comprising:
- combining a plurality of living cells, a plurality of fluorescent molecules, and a plurality of cyclodextrin molecules in an aqueous solution;
- and
- emulsifying the aqueous solution with a hydrophobic fluid to form the aqueous droplets;
- wherein at least one of the aqueous droplets includes (i) a single living cell or a single cell species in a homogenous aggregate of the plurality of living cells, (ii) at least one fluorescent molecule of the plurality of fluorescent molecules, and (iii) at least one cyclodextrin molecule of the plurality of cyclodextrin molecules.
An emulsion comprising a plurality of aqueous droplets suspended in a hydrophobic fluid, wherein at least one of the aqueous droplets includes:
- a single living cell or a single cell species in a homogenous aggregate;
- at least one fluorescent molecule;
- and
- at least one cyclodextrin molecule.
Dependencies are inferred from the claim text.