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US 2024/0401002 A1Published application
Novel flavin-dependent lactate dehydrogenase and method for improving stability of lactate dehydrogenase
AssigneeKikkoman Corporation
InventorsRyota SUZUKI, Atsushi ICHIYANAGI
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Abstract
The present invention relates to a novel flavin-dependent lactate dehydrogenase and a method for improving stability of lactate dehydrogenase.
Claims10
Lactate dehydrogenase which comprises an amino acid sequence selected from the following (i) to (iii):
- (i) an amino acid sequence represented by SEQ ID NO:
- 3,
- (ii) an amino acid sequence having 70% or more identify with the amino acid sequence represented by SEQ ID NO:
- 3, or
- (iii) when an alignment with the amino acid sequence represented by SEQ ID NO:
- 3 is formed, an amino acid sequence having 70% or more identify with the amino acid sequence represented by SEQ ID NO:
- 3 in a region from positions 110 to 502 of SEQ ID NO:
- 3, and
- containing deletion of an N-terminal, and/or mutation(s) at one or more positions selected from the group consisting of position 54, position 156, position 349 and position 428.
6 more elements
A method for improving stability of lactate dehydrogenase, which comprises the steps of deleting an N-terminal and/or mutating at one or more positions selected from the group consisting of position 54, position 156, position 349 and position 428 of the lactate dehydrogenase having an amino acid sequence selected from the following (i) to (iii):
- (i) the amino acid sequence represented by SEQ ID NO:
- 3,
- (ii) an amino acid sequence having 70% or more identify with the amino acid sequence represented by SEQ ID NO:
- 3, or
- (iii) when an alignment with the amino acid sequence represented by SEQ ID NO:
- 3 is formed, an amino acid sequence having 70% or more identify with the amino acid sequence represented by SEQ ID NO:
- 3 in the region from positions 110 to 502 of SEQ ID NO:
- 3.
5 more elements
Dependencies are inferred from the claim text.