Feline leukemia virus vaccine

A method comprising:

• (a) administering a primer vaccine to a feline subject, the primer vaccine comprising a first Venezuelan Equine Encephalitis (VEE) alphavirus RNA replicon particle (RP) that encodes a first feline leukemia virus (FeLV) glycoprotein 85 (gp85) or an antigenic fragment thereof, and a first pharmaceutically acceptable carrier, and
• (b) administering a booster vaccine to the feline subject, the booster vaccine comprising a second VEE alphavirus RNA RP that encodes a second FeLV gp85 or an antigenic fragment thereof, and a second pharmaceutically acceptable carrier.

An alpha RNA replicon particle (RP) comprising a heterologous nucleic acid sequence, the heterologous nucleic acid sequence having at least 95% sequence identity to SEQ ID NO:

• 1 or SEQ ID NO:10, wherein the RP is a Venezuelan Equine Encephalitis (VEE) RP.