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US 2024/0398294 A1Published application
Disposable electrochemical biosensor based on NAD(P)-dependent dehydrogenase and diaphorase
AssigneeNova Biomedical Corporation
InventorsJianhong Pei, Joseph Bedard, Aaron Tram, Samantha Mosley, Chung Chang Young
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Abstract
A disposable biosensor that includes a sensing surface having at least a working electrode portion and a reference electrode portion, a first reagent disposed on the working electrode portion containing a NAD(P)-dependent dehydrogenase, NAD(P)+, diaphorase, and an oxidized form of a redox mediator forming a working electrode, and a reference electrode material disposed on the reference electrode portion forming a reference electrode.
Claims20
A biosensor comprising:
- a sensing surface comprising a working electrode portion and a blank electrode portion;
- a first soluble reagent disposed on the working electrode portion, wherein the first soluble reagent comprises a NAD(P)-dependent dehydrogenase, NAD(P)+, diaphorase, and an oxidized form of a redox mediator;
- and
- a second soluble reagent disposed on the blank electrode portion, wherein the second soluble reagent does not contain dehydrogenase;
- wherein the biosensor is configured such that the first soluble reagent dissolves while a sample fills a sample chamber of the biosensor, at least in part, by capillary action.
2 more elements
A method of making a biosensor, the method comprising:
- disposing a first soluble reagent on a first portion of the biosensor, wherein the first soluble reagent comprises a NAD(P)-dependent dehydrogenase, NAD(P)+, diaphorase, and an oxidized form of a redox mediator, and wherein the first soluble reagent is configured to dissolve while a sample fills a sample chamber of the biosensor, at least in part, by capillary action;
- and
- disposing a second soluble reagent on a second portion of the biosensor, wherein the second soluble reagent does not contain dehydrogenase.
Dependencies are inferred from the claim text.