Method and system for early detection of bovine mastitis using enhanced chemiluminescence

Abstract

A method and system for early detection of Bovine Mastitis (BM) uses enhanced chemiluminescence (CL) to determine Haptoglobin (Hp) levels in highly diluted milk samples. The samples are placed in the wells of one or more bio-functionalized Hemoglobin (Hb) modified CL assay plates. Hp-Hb binding occurs in those samples containing Hp. After a first pre-determined time duration, the wells are treated with a CL solution containing luminol and peroxide, and a colloidal suspension of crosslinked nanoparticles. After a second pre-determined time duration, the CL intensity approaches a steady state value. A processor analyzes CL images provided by a camera in order to measure CL intensity and to determine an estimate of Hp level in each well, based on a pre-determined regression curve. The processor then forms a BM clinical diagnosis by combining the estimated HP levels from wells with different milk sample dilutions.

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