Methods for detecting heavy metals in biological samples
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsMary PRUNICKI, Kari C. NADEAU, Eric M. SMITH
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Abstract
The present disclosure provides methods for detecting heavy metals in tissues and cells while identifying cell types at the single-cell level with intracellular or cell surface heavy metal content. The cell type can be identified by contacting the cell with an antibody that binds a biomarker conjugated to a heavy metal that is different from the heavy metal detected in or on the surface of the cell. The methods include detecting heavy metals using mass cytometry.
Claims22
A method for detecting an amount of one or more heavy metals in a biological sample, the method comprising:
- (a) contacting the biological sample with one or more antibodies conjugated to a heavy metal;
- and
- (b) detecting both the amount of heavy metals conjugated to the one or more antibodies and the amount of the one or more heavy metals in the biological sample, wherein the one or more heavy metals in the biological sample are not conjugated to an antibody.
Dependencies are inferred from the claim text.