Electrochemical Aptamer Sensors With Stable Blocking Layers, Rapid Electron Transfer And Robust Antifouling Properties
AssigneeUniversity of Cincinnati
InventorJason Charles Heikenfeld
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Abstract
The present invention relates to a device for continuous sensing of at least one analyte in a test fluid that is resistant at least one of desorption of a first plurality of molecules, including a plurality of aptamers and a blocking layer, and fouling during use of the device when exposed to temperatures greater than or equal to 30° C. for at least 3 days. Another aspect of the invention is a method of providing fouling resistance to an aptamer sensor device wherein the blocking layer and aptamer are resistant to at least one of desorption from the electrode and fouling during use of the aptamer sensor when exposed to temperatures greater than or equal to 30° C. for at least 3 days.
Claims82
A device for continuous sensing of at least one analyte in a test fluid, comprising:
- an electrode comprising an electrode surface;
- a first plurality of molecules, comprising:
- a plurality of aptamers comprising attached redox tags, wherein the attached redox tags provide electron transfer with the electrode;
- and
- a blocking layer formed on the electrode, wherein the blocking layer comprises a blocking layer surface and a plurality of features supporting the electron transfer between the electrode and the redox tags;
- and
- wherein the first plurality of molecules, when exposed to temperatures greater than or equal to 30° C., is resistant to at least one of desorption from the electrode and fouling during use of the device for at least 3 days.
A method of providing fouling resistance to an aptamer sensor, the aptamer sensor including an electrode having an electrode surface and an aptamer having a redox tag, the method comprising:
- binding a plurality of blocking molecules to the electrode to form a blocking layer having a blocking layer surface,
- wherein the blocking layer has a plurality of features supporting electron transfer between the electrode and the redox tag, and wherein the blocking layer and the aptamer are resistant to at least one of desorption from the electrode and fouling during use of the aptamer sensor when exposed to test fluid and temperatures greater than or equal to 30° C. for at least 3 days;
- and
- wherein the aptamer is attached to a surface selected from the group consisting of the electrode surface and the blocking layer surface.
Dependencies are inferred from the claim text.