US 2024/0393246 A1 · SYSTEMS AND METHODS OF BACTERIAL GROWTH DETECTION USING DUAL LIGHT EXCITATION

Abstract

A method of determining the presence of an analyte of interest in a blood sample within a test device including a sensor in aqueous media includes transmitting light to the test device at a first excitation wavelength at which light absorption of the sensor remains substantially constant as pH of the aqueous media changes, measuring an intensity of a first fluorescence signal emitted from the sensor in the test device in response to the first excitation wavelength, transmitting light to the test device at a second excitation wavelength, the second excitation wavelength being different from the first excitation wavelength, measuring an intensity of a second fluorescence signal emitted from the sensor in the test device in response to the second excitation wavelength, and normalizing, with the first fluorescence signal, the intensity of the second fluorescence signal emitted from the test device in response to the second excitation wavelength.

Claims28

Dependent claims

A method of determining the presence of an analyte of interest in a blood sample within a test device comprising a sensor in aqueous media, the method comprising:

transmitting light to the test device at a first excitation wavelength at which a light absorption of the sensor remains substantially constant as a pH of the aqueous media changes;
measuring an intensity of a first fluorescence signal emitted from the sensor in the test device in response to the first excitation wavelength;
transmitting light to the test device at a second excitation wavelength, the second excitation wavelength being different from the first excitation wavelength;
measuring an intensity of a second fluorescence signal emitted from the sensor in the test device in response to the second excitation wavelength;
and
normalizing, with the first fluorescence signal, the intensity of the second fluorescence signal emitted from the test device in response to the second excitation wavelength.

3 more elements

A system for determining the presence of an analyte of interest in a blood sample, the system comprising:

a blood culture test device comprising a sensor in an aqueous media and configured to receive a blood sample;
a first light source;
a first excitation filter, wherein the first excitation filter is configured to filter light from the first light source to provide light to the sensor at a first excitation wavelength at which a light absorption of the sensor remains substantially constant as a pH of the aqueous media changes;
a second light source;
a second excitation filter, wherein the second excitation filter is configured to filter light from the second light source to provide light to the sensor at a second excitation wavelength, the second excitation wavelength being different from the first excitation wavelength;
one or more detectors configured to measure an intensity of a first fluorescence signal emitted from the sensor in the test device in response to the first excitation wavelength and an intensity of a second fluorescence signal emitted from the sensor in the test device in response to the second excitation wavelength;
and
a processor configured to normalize the measurement of the intensity of the second fluorescence signal emitted from the sensor in the test device in response to the second excitation wavelength using the measurement of the intensity of the first fluorescence signal emitted from the sensor in the test device in response to the first excitation wavelength.

5 more elements

Dependencies are inferred from the claim text.

Systems and methods of bacterial growth detection using dual light excitation

Life of this patent

Systems and methods of bacterial growth detection using dual light excitation

Life of this patent

Abstract

Claims28